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The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents

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Book cover Pre-Clinical Models

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

The elevated plus maze test is used to measure anxiety-like behavior in rodents. It can be used to gain insight into conditions such as posttraumatic stress disorder (PTSD) and other conditions marked by anxious behavior. It can also be used as a component in screening of novel compounds for anxiolytic properties. This model is based on aversion to open spaces, which is seen as the animal spending more time in the enclosed arms of the maze. This chapter describes the steps necessary for setting up and conducting the test, along with interpretation of the results.

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References

  1. Sarnyai Z, Alsaif M, Bahn S, Ernst A, Guest PC, Hradetzky E et al (2011) Behavioral and molecular biomarkers in translational animal models for neuropsychiatric disorders. Int Rev Neurobiol 101:203–238

    Article  CAS  PubMed  Google Scholar 

  2. Crawley JN (2004) Designing mouse behavioral tasks relevant to autistic-like behaviors. Ment Retard Dev Disabil Res Rev 10(4):248–258

    Article  PubMed  Google Scholar 

  3. Nestler EJ, Hyman SE (2010) Animal models of neuropsychiatric disorders. Nat Neurosci 13(10):1161–1169

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  4. Kraeuter AK, Guest PC, Sarnyai Z (2018) Neuropsychiatric sequelae of early nutritional modifications: a Beginner’s guide to behavioral analysis. Methods Mol Biol 1735:403–420

    Article  CAS  PubMed  Google Scholar 

  5. Handley SL, Mithani S (1984) Effects of alpha-adrenoceptor agonists and antagonists in a maze-exploration model of ‘fear’-motivated behaviour. Naunyn Schmiedeberg’s Arch Pharmacol 327(1):1–5

    Article  CAS  Google Scholar 

  6. File SE (1987) The contribution of behavioural studies to the neuropharmacology of anxiety. Neuropharmacology 26(7B):877–886

    Article  CAS  PubMed  Google Scholar 

  7. McGonigle P (2014) Animal models of CNS disorders. Biochem Pharmacol 87(1):140–149

    Article  CAS  PubMed  Google Scholar 

  8. Ennaceur A, Chazot PL (2016) Preclinical animal anxiety research - flaws and prejudices. Pharmacol Res Perspect 4(2):e00223. https://doi.org/10.1002/prp2.223

    Article  PubMed  PubMed Central  Google Scholar 

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Author information

Authors and Affiliations

  1. Laboratory of Psychiatric Neuroscience, Australian Institute of Tropical Health and Medicine, James Cook University, Townsville, QLD, Australia

    Ann-Katrin Kraeuter & Zoltán Sarnyai

  2. Discipline of Biomedicine, College of Public Health, Medicine and Veterinary Sciences, James Cook University, Townsville, QLD, Australia

    Ann-Katrin Kraeuter & Zoltán Sarnyai

  3. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, SP, Brazil

    Paul C. Guest

Authors
  1. Ann-Katrin Kraeuter
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  2. Paul C. Guest
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  3. Zoltán Sarnyai
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Corresponding author

Correspondence to Zoltán Sarnyai .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Kraeuter, AK., Guest, P.C., Sarnyai, Z. (2019). The Elevated Plus Maze Test for Measuring Anxiety-Like Behavior in Rodents. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_4

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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