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A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue

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Pre-Clinical Models

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

Here we describe a mass spectrometry-based proteomics workflow to discovery proteins differentially regulated in brains collected postmortem from mental, neurological, or substance abuse disorders (MNS) patients. One way to maximize protein detection is to carry out enrichment of cellular compartments such as the nucleus, mitochondria and cytosol. Subcellular fractionation improves proteome coverage and may shed light on the role of these organelles in the pathophysiology of MNS.

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Author information

Authors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, Brazil

    Guilherme Reis-de-Oliveira, Mariana Fioramonte & Daniel Martins-de-Souza

  2. Center for Neurobiology, University of Campinas (UNICAMP), Campinas, Brazil

    Daniel Martins-de-Souza

  3. Instituto Nacional de Biomarcadores em Neuropsiquiatria (INBION), Conselho Nacional de Desenvolvimento Cientifico e Tecnologico, Sao Paulo, Brasil

    Daniel Martins-de-Souza

Authors
  1. Guilherme Reis-de-Oliveira
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  2. Mariana Fioramonte
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  3. Daniel Martins-de-Souza
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Corresponding author

Correspondence to Daniel Martins-de-Souza .

Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Reis-de-Oliveira, G., Fioramonte, M., Martins-de-Souza, D. (2019). A Complete Proteomic Workflow to Study Brain-Related Disorders via Postmortem Tissue. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_31

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_31

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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