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OVA-Induced Allergic Airway Inflammation Mouse Model

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Pre-Clinical Models

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1916))

Abstract

Asthma is a worldwide public health issue, affecting the sufferer’s quality of life. Many researchers are extensively studying the cellular processes involved in the affected airways. Experimental asthma using animals has been performed for a long time, mainly applying murine models due to well-known advantages. The aim of this study is to present an allergic airway inflammation protocol in mice. Basically, the allergic airway inflammation is induced by intraperitoneal sensitization and intratracheal challenge with ovalbumin (OVA). The model provided here mimics acute asthma characteristics including excessive mucus production, airway hyperresponsiveness, and eosinophilic airway inflammation.

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References

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Acknowledgments

This study was funded by São Paulo Research Foundation (FAPESP), project number 2012/50410-8 to CMF; 2012/50336-2 and 2016/50484-2 to FAO. CNPq (The National Council for Scientific and Technological Development), project number 486037/2012-6 to CMF. Also, the authors would like to acknowledge the fellowships provided by the Coordenação de Aperfeiçoamento Pessoal de Nível Superior - Brasil (CAPES) -Finance Code 001 to VRS and São Paulo Research Foundation (FAPESP), project number 2016/13496-2 to MCC.

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Author notes

  1. Mateus Casaro and Vanessa R. Souza contributed equally to this work.

Authors and Affiliations

  1. Department of Pharmaceutical Sciences, Institute of Environmental, Chemistry and Pharmaceutical Sciences, Universidade Federal de São Paulo, Diadema, SP, Brazil

    Mateus Casaro, Vanessa R. Souza & Caroline M. Ferreira

  2. Cellular and Molecular Neurobiology Laboratory (LaNeC), Center for Mathematics, Computing and Cognition (CMCC), Federal University of ABC–UFABC, São Bernardo do Campo, SP, Brazil

    Fernando A. Oliveira

  3. Department of Neuroscience, Carleton University, Ottawa, ON, Canada

    Fernando A. Oliveira

Authors
  1. Mateus Casaro
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  2. Vanessa R. Souza
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  3. Fernando A. Oliveira
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  4. Caroline M. Ferreira
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Editor information

Editors and Affiliations

  1. Laboratory of Neuroproteomics, Department of Biochemistry and Tissue Biology, Institute of Biology, University of Campinas (UNICAMP), Campinas, São Paulo, Brazil

    Paul C. Guest

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Casaro, M., Souza, V.R., Oliveira, F.A., Ferreira, C.M. (2019). OVA-Induced Allergic Airway Inflammation Mouse Model. In: Guest, P. (eds) Pre-Clinical Models. Methods in Molecular Biology, vol 1916. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8994-2_28

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  • DOI: https://doi.org/10.1007/978-1-4939-8994-2_28

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8993-5

  • Online ISBN: 978-1-4939-8994-2

  • eBook Packages: Springer Protocols

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