Plant Genome Editing with CRISPR Systems pp 97-107 | Cite as
Generating Photoperiod-Sensitive Genic Male Sterile Rice Lines with CRISPR/Cas9
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Abstract
Obtaining photoperiod-sensitive genic male sterility (PGMS) lines is one of the most important steps in two-line hybrid rice breeding. Traditionally, such lines were screened and developed with a classic rice breeding system under both long-day and short-day conditions. The isolation and backcross process used for this could easily last for more than 3 years with a very low success rate. Here, we describe a straightforward method for generating csa-based PGMS lines by using the CRISPR-Cas9 technology in rice.
Key words
Photoperiod-sensitive genic male sterility (PGMS) Two-line hybrid rice Carbon starved anther (csa) CRISPR-Cas9 Agrobacterium-mediated transformation Transgene-freeNotes
Acknowledgments
This work was supported by the funds from the National Transgenic Major Program (2016ZX08009003-003-007) and National Key Research and Development Program of China (2016YFD0101107) to ZY.
References
- 1.Cheng SH, Zhuang JY, Fan YY, Du JH, Cao LY (2007) Progress in research and development on hybrid rice: a super-domesticate in China. Ann Bot 100:959–966CrossRefGoogle Scholar
- 2.Mou T, Cao B, Huang Z, Yang G, Lin Y, Xiang G (2001) Characterization of fertility alteration in 8 environment-sensitive genic male-sterile lines of indica rice. Acta Bot Sin 43:238–242Google Scholar
- 3.Zhang HL, Chen XY, Huang JZ, Zhi Guo E, Gong JY, Shu QY (2015) Identification and transition analysis of photo-/thermo-sensitive genic male sterile genes in two-line hybrid rice in China. Sci Agric Sin 31:175–182Google Scholar
- 4.Woo M, Ham T, Ji H, Choi M, Jiang W, Chu S, Piao R, Chin J, Kim J, Park B (2008) Inactivation of the UGPase1 gene causes genic male sterility and endosperm chalkiness in rice (Oryza sativa L.). Plant J 54:190CrossRefGoogle Scholar
- 5.Li Q, Zhang D, Chen M, Liang W, Wei J, Qi Y, Yuan Z (2016) Development of japonica photo-sensitive genic male sterile rice lines by editing carbon starved anther using CRISPR/Cas9. J Genet Genomics 43:415–419CrossRefGoogle Scholar
- 6.Zhang H, Liang W, Yang X, Luo X, Jiang N, Ma H, Zhang D (2010) Carbon starved anther encodes a MYB domain protein that regulates sugar partitioning required for rice pollen development. Plant Cell 22:672CrossRefGoogle Scholar
- 7.Zhang H, Xu C, He Y, Zong J, Yang X, Si H, Sun Z, Hu J, Liang W, Zhang D (2013) Mutation in CSA creates a new photoperiod-sensitive genic male sterile line applicable for hybrid rice seed production. Proc Natl Acad Sci U S A 110:76–81CrossRefGoogle Scholar
- 8.Zhang H, Zhang J, Wei P, Zhang B, Gou F, Feng Z, Mao Y, Yang L, Zhang H, Xu N, Zhu JK (2014) The crispr/cas9 system produces specific and homozygous targeted gene editing in rice in one generation. Plant Biotechnology Journal, 12, 797–807.CrossRefGoogle Scholar
- 9.Mao Y, Zhang H, Xu N, Zhang B, Gou F, Zhu JK (2013) Application of the CRISPR–Cas system for efficient genome engineering in plants. Mol Plant 6:2008CrossRefGoogle Scholar
- 10.Nishimura A, Aichi I, Matsuoka M (2006) A protocol for Agrobacterium-mediated transformation in rice. Nat Protoc 1:2796–2802CrossRefGoogle Scholar
- 11.Ozawa K (2012) A high-efficiency Agrobacterium-mediated transformation system of rice (Oryza sativa L.). Methods Mol Biol 847:51–57CrossRefGoogle Scholar
- 12.Zhang DB, Wilson ZA (2009) Stamen specification and anther development in rice. Chin Sci Bull 54:2342–2353CrossRefGoogle Scholar