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Mutagenesis Screens for Prostate Cancer Using Replication-Incompetent Lentiviral Vectors

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Cancer Driver Genes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1907))

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Abstract

Prostate cancer (PC) is the second leading cause of cancer-related deaths in US men, and progression to androgen-independent PC (AIPC) typically results in metastasis and is lethal. However, the mechanisms whereby PC progresses from androgen dependence to androgen independence are not completely understood. Mutagenesis screens to identify novel genes involved in the progression to AIPC have been performed using replication-incompetent lentiviral vectors (LVs). In this approach the LV acts both as a mutagen and as molecular tag to identify nearby genes that may have been dysregulated by the vector provirus, and are candidate AIPC genes. Here we describe protocols for generation of replication-incompetent LV preparations and performing a mutagenesis screen to identify AIPC genes in vitro.

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Acknowledgment

This work was supported by NIH grants AI097100 and AI102672.

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Authors and Affiliations

  1. Pharmaceutical Sciences, Washington State University, Spokane, WA, USA

    Grant D. Trobridge

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  1. Grant D. Trobridge
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Corresponding author

Correspondence to Grant D. Trobridge .

Editor information

Editors and Affiliations

  1. Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, MN, USA

    Timothy K. Starr

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Trobridge, G.D. (2019). Mutagenesis Screens for Prostate Cancer Using Replication-Incompetent Lentiviral Vectors. In: Starr, T. (eds) Cancer Driver Genes. Methods in Molecular Biology, vol 1907. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8967-6_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8967-6_8

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8966-9

  • Online ISBN: 978-1-4939-8967-6

  • eBook Packages: Springer Protocols

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