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Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization

Part of the Methods in Molecular Biology book series (MIMB,volume 1907)

Abstract

The tissue engineering method of decellularization and recellularization has been successfully used in a variety of regenerative medicine applications. The protocols used to de/recellularize various organs and tissues are largely different. Here we describe a method to effectively engineer a bioartificial colon by completely removing original cells from human intestinal tissues followed by repopulating the acellular tissue matrix with cell cultures. This method provides a novel approach for human intestinal regeneration and can be used to identify potential cancer driver genes.

Key words

  • Decellularization
  • Recellularization
  • Tissue engineering
  • Human intestine
  • Disease modeling

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Acknowledgment

This work was supported by NCI-PSOC Young Investigator trans-network grant (to H.J.C.), Arnold O. Beckman Postdoctoral fellowship (to H.J.C), NIH-UH2TR000516 (to M.L.S.), and NSF-1106153 (to M.L.S.).

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Correspondence to Michael L. Shuler .

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Chen, H.J., Shuler, M.L. (2019). Engineering a Bioartificial Human Colon Model Through Decellularization and Recellularization. In: Starr, T. (eds) Cancer Driver Genes. Methods in Molecular Biology, vol 1907. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8967-6_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8967-6_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8966-9

  • Online ISBN: 978-1-4939-8967-6

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