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Liver-Specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation

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Cancer Driver Genes

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1907))

Abstract

Understanding the complex genetic background of cancers is key in developing effective targeted therapies. The Sleeping Beauty (SB) transposon system is a powerful and unbiased genetic editing tool that can be used for rapid screening of candidate liver cancer driver genes. Manipulating their expression level using a reverse genetic mouse model involving hydrodynamic tail-vein injection delivery can rapidly elucidate the role of these candidate genes in liver cancer tumorigenesis.

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Author information

Authors and Affiliations

  1. Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Kowloon, Hong Kong, China

    Amy P. Chiu & Vincent W. Keng

Authors
  1. Amy P. Chiu
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  2. Vincent W. Keng
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Corresponding author

Correspondence to Vincent W. Keng .

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Editors and Affiliations

  1. Masonic Cancer Center, University of Minnesota Medical School, Minneapolis, MN, USA

    Timothy K. Starr

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Chiu, A.P., Keng, V.W. (2019). Liver-Specific Delivery of Sleeping Beauty Transposon System by Hydrodynamic Injection for Cancer Gene Validation. In: Starr, T. (eds) Cancer Driver Genes. Methods in Molecular Biology, vol 1907. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8967-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8967-6_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8966-9

  • Online ISBN: 978-1-4939-8967-6

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