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Sample Preconcentration Protocols in Microfluidic Electrophoresis

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Microfluidic Electrophoresis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1906))

Abstract

Electrophoretic on-line sample preconcentration techniques in microfluidic channels improve the sensitivity prior to the separation. Among various techniques, the most important field-amplified sample stacking and sweeping on cross-channel microchips are demonstrated. As a novel microfluidic preconcentration approach, a large-volume sample stacking with electroosmotic flow pump (LVSEP) on straight-channel chips is also presented, which can omit a complicated voltage program for sample injection processes. In this chapter, we describe how to prepare and how to run these on-line sample preconcentration methods in microchip electrophoresis.

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References

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Acknowledgments

We would like to thank Dr. Kenji Sueyoshi (Osaka Prefecture University, Japan) and Dr. Takayuki Kawai (RIKEN, Japan) for collaborating the sweeping and LVSEP experiments, respectively. This work was supported in part by the Grant-in-Aid for Scientific Research (C) (No. 24550090 and 15K05527) from the Japan Society for the Promotion of Science (JSPS). This research was also supported by SENTAN, JST.

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Authors and Affiliations

  1. Department of Frontier Materials Chemistry, Graduate School of Science and Technology, Hirosaki University, Hirosaki, Japan

    Fumihiko Kitagawa

  2. Department of Material Chemistry, Graduate School of Engineering, Kyoto University, Kyoto, Japan

    Koji Otsuka

Authors
  1. Fumihiko Kitagawa
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  2. Koji Otsuka
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Corresponding author

Correspondence to Fumihiko Kitagawa .

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Editors and Affiliations

  1. Department of Chemistry, University of Wyoming, Laramie, WY, USA

    Debashis Dutta

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Kitagawa, F., Otsuka, K. (2019). Sample Preconcentration Protocols in Microfluidic Electrophoresis. In: Dutta, D. (eds) Microfluidic Electrophoresis. Methods in Molecular Biology, vol 1906. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8964-5_4

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  • DOI: https://doi.org/10.1007/978-1-4939-8964-5_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8963-8

  • Online ISBN: 978-1-4939-8964-5

  • eBook Packages: Springer Protocols

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