Skip to main content

Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device

Part of the Methods in Molecular Biology book series (MIMB,volume 1905)

Abstract

Construction of three-dimensional (3D) hepatic tissue structures is important for in vitro tissue engineering of the liver, because 3D culture of hepatocytes is critical for the maintenance of liver-specific functions. Although conventional 3D culture methods are useful for constructing 3D hepatic tissue structures, the precise control of culture microenvironments is required to construct more physiological tissues in vitro. Recent advances in microfluidics technologies have allowed us to utilize microfluidic devices for hepatic cell culture, which opened the door for creating more physiological 3D culture models of the liver. Here, we describe the method for the construction of hepatic tissue structures using a microfluidic device which has a 3D gel region with adjacent microchannels. Primary rat hepatocytes are seeded into a microchannel in a microfluidic device. The cells are then cultured in interstitial flow conditions, which leads to the construction of 3D tissue structures.

Key words

  • Microfluidic device
  • Interstitial flow
  • 3D culture

This is a preview of subscription content, access via your institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-1-4939-8961-4_15
  • Chapter length: 8 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   169.00
Price excludes VAT (USA)
  • ISBN: 978-1-4939-8961-4
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Hardcover Book
USD   219.99
Price excludes VAT (USA)
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Sudo R (2014) Multiscale tissue engineering for liver reconstruction. Organogenesis 10:216–224

    CrossRef  PubMed  PubMed Central  Google Scholar 

  2. Chung S, Sudo R, Vickerman V, Zervantonakis IK, Kamm RD (2010) Microfluidic platforms for studies of angiogenesis, cell migration, and cell-cell interactions. Ann Biomed Eng 38:1164–1177

    CrossRef  PubMed  Google Scholar 

  3. Zervantonakis IK, Kothapalli CR, Chung S, Sudo R, Kamm RD (2011) Microfluidic devices for studying heterotypic cell-cell interactions and tissue specimen cultures under controlled microenvironments. Biomicrofluidics 5:13406

    CrossRef  PubMed  Google Scholar 

  4. Shin Y, Han S, Jeon JS, Yamamoto K, Zervantonakis IK, Sudo R, Kamm RD, Chung S (2012) Microfluidic assay for simultaneous culture of multiple cell types on surfaces or within hydrogels. Nat Protoc 7:1247–1259

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  5. Sudo R, Chung S, Zervantonakis IK, Vickerman V, Toshimitsu Y, Griffith LG, Kamm RD (2009) Transport-mediated angiogenesis in 3D epithelial coculture. FASEB J 23:2155–2164

    CAS  CrossRef  PubMed  PubMed Central  Google Scholar 

  6. Yamamura N, Sudo R, Ikeda M, Tanishita K (2007) Effects of the mechanical properties of collagen gel on the in vitro formation of microvessel networks by endothelial cells. Tissue Eng 13:1443–1453

    CAS  CrossRef  PubMed  Google Scholar 

  7. Chung S, Sudo R, Mack PJ, Wan CR, Vickerman V, Kamm RD (2009) Cell migration into scaffolds under co-culture conditions in a microfluidic platform. Lab Chip 9:269–275

    CAS  CrossRef  PubMed  Google Scholar 

  8. Chung S, Sudo R, Zervantonakis IK, Rimchala T, Kamm RD (2009) Surface treatment induced three dimensional capillary morphogenesis in a microfluidic platform. Adv Mater 21:4863–4867

    CAS  CrossRef  PubMed  Google Scholar 

Download references

Acknowledgment

This work was supported by JSPS KAKENHI Grant Number 16H03173 to R.S.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ryo Sudo .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 2019 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Verify currency and authenticity via CrossMark

Cite this protocol

Sudo, R. (2019). Reconstruction of Hepatic Tissue Structures Using Interstitial Flow in a Microfluidic Device. In: Tanimizu, N. (eds) Hepatic Stem Cells . Methods in Molecular Biology, vol 1905. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8961-4_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-8961-4_15

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8960-7

  • Online ISBN: 978-1-4939-8961-4

  • eBook Packages: Springer Protocols