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Human Monoclonal Antibodies pp 189–212Cite as

One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1904))

Abstract

Recent improvements in the flow cytometry technology allow the determination of the general immune status through the development of multicolor immunofluorescence panels. The one-tube multicolor flow cytometry assay (OTMA) that is presented here identifies 20 different, clinically relevant immune cell subsets and three common activation markers. Thereby, a comprehensive immune status that covers all major immune cells is easily obtained.

The assay is suitable for every common three lasers and 10 color flow cytometer and includes the application of 15 different antibodies. Furthermore, the assay requires only 100 μL of EDTA-treated whole-blood and less than 40 min for sample preparation. By being easily adaptable to individual requirements and by additionally determining absolute cell counts, the assay is well-suited for translational research in clinical trials.

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Author notes

  1. Anna-Jasmina Donaubauer and Paul F. Rühle contributed equally to this work. Udo S. Gaipl and Benjamin Frey contributed equally as senior authors.

Authors and Affiliations

  1. Department of Radiation Oncology, Universitätsklinikum Erlangen, Friedrich-Alexander-Universität Erlangen-Nürnberg, Erlangen, Germany

    Anna-Jasmina Donaubauer, Paul F. Rühle, Ina Becker, Rainer Fietkau, Udo S. Gaipl & Benjamin Frey

Authors
  1. Anna-Jasmina Donaubauer
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  2. Paul F. Rühle
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  3. Ina Becker
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  4. Rainer Fietkau
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  5. Udo S. Gaipl
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  6. Benjamin Frey
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Corresponding author

Correspondence to Udo S. Gaipl .

Editor information

Editors and Affiliations

  1. Department of Pathology, The Hebrew University, Hadassah Medical School, Jerusalem, Israel

    Michael Steinitz

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Donaubauer, AJ., Rühle, P.F., Becker, I., Fietkau, R., Gaipl, U.S., Frey, B. (2019). One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood. In: Steinitz, M. (eds) Human Monoclonal Antibodies. Methods in Molecular Biology, vol 1904. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8958-4_8

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  • DOI: https://doi.org/10.1007/978-1-4939-8958-4_8

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8957-7

  • Online ISBN: 978-1-4939-8958-4

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