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Inheritance of Transgenes in Transgenic Bt Lines Resistance to Helicoverpa armigera in Upland Cotton

  • Baolong Zhang
  • Wangzhen Guo
  • Tianzhen ZhangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1902)

Abstract

Six transgenic Bt cotton cultivars (lines) including GKsu12, GK19, MR1, GK5, 109B, and SGK1 are highly resistant to bollworm from the seedling to boll-setting stages in bioassays with detached cotton leaves, though there are differences in resistant level and Bt toxin content in these transgenic cottons. Genetics analysis reveals that the resistance to Helicoverpa armigera in these six transgenic Bt cotton cultivars (lines) are controlled by one pair of dominant genes. Allelic tests further demonstrate some populations are in Mendel segregation for two nonallelic genes, i.e., the inserted Bt gene in GKsu12 is nonallelic to that of SGK1, GK5, 109B, and GK19, and Bt genes in GK19 and SGK1 are likely inserted in the same or in close proximity (genetically closely linked), while some F2 produce abnormal segregation patterns, with a segregation of resistance to Helicoverpa armigera which vary between 15:1 and 3:1, though their Bt segregation fit into 15:1 by PCR analysis, suggesting Bt gene silence in these populations. Two genes silence may occur in these populations due to the homologous sequence by crossing since the silenced individuals accounted for 1/16 of the F2 populations for allelic test. To those silenced populations, one of their parents all showed high resistance to bollworm.

Key words

Transgenic Bt cotton Insect resistance Helicoverpa armigera Gene silence 

Notes

Acknowledgments

We thank Dr. John Z Yu, USDA-ARS, Southern Plains Agriculture Research Center, Crop Germplasm Research Unit, USA, for the critical reviewing of this manuscript. This project was financially supported in part by the National Transgenic R&D Project in China (2011ZX08005-001) and the Priority Academic Program Development of Jiangsu Higher Education Institutions.

References

  1. 1.
    Hofte H, Whiteley HR (1989) Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol Rev 53:242–255PubMedPubMedCentralGoogle Scholar
  2. 2.
    Benedict JH, Sachs ES, Altman DW, Ring DR, Stone TB, Sims SR (1993) Impact of endotoxin-producing transgenic cotton on insect- plant interaction with Heliothis virescens and Helicoverpa zea (Lepidoptera: Noctuidae). Environ Entomol 22:1–9CrossRefGoogle Scholar
  3. 3.
    Clive J (2010) Global status of commercialized biotech/GM crops: 2010. ISAAA Brief No.42. ISAAA, Ithaca, NYGoogle Scholar
  4. 4.
    Sun J, Tang CM, Zhu XF, Guo WZ, Zhang TZ, Zhou WJ, Meng FX, Sheng JL (2002) Characterization of resistance to Helicoverpa armigera in three lines of transgenic Bt Upland cotton. Euphytica 123:343–351CrossRefGoogle Scholar
  5. 5.
    Wu K, Lu Y, Feng H, Jiang Y, Zhao J (2008) Suppression of cotton bollworm in multiple crops in China in areas with Bt toxin-containing cotton. Science 321:1676–1678CrossRefGoogle Scholar
  6. 6.
    Dong H, Li W (2007) Variability of endotoxin expression in Bt transgenic cotton. J Agron Crop Sci 193:21–29CrossRefGoogle Scholar
  7. 7.
    Tabashnik BE, Sisterson MS, Ellsworth PC, Dennehy TJ, Antilla L, Liesner L, Whitlow M, Staten RT, Fabrick JA, Unnithan GC (2010) Suppressing resistance to Bt cotton with sterile insect releases. Nat Biotechnol 28:1304–1307CrossRefGoogle Scholar
  8. 8.
    Register JC, Peterson DJ, Bell PJ, Bullock WP, Evans IJ, Frame B, Greenland AJ, Higgs NS, Jepson I, Jiao S, Lewnau CJ, Sillick JM, Wilson HM (1994) Structure and function of selectable and non-selectable transgenes in maize after introduction by particle bombardment. Plant Mol Biol 25:951–961CrossRefGoogle Scholar
  9. 9.
    Cheng M, Fry JE, Pang S, Zhou H, Hironaka CM, Duncan DR, Conner TW, Wan Y (1997) Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol 115:971–980CrossRefGoogle Scholar
  10. 10.
    Spencer TM, O’Brien JV, Start WG, Adams TR, Gordon-Kamm WJ, Lemaux PG (1992) Segregation of transgenes in maize. Plant Mol Biol 18:201–210CrossRefGoogle Scholar
  11. 11.
    Pawlowski WP, Somers DA (1996) Transgene inheritance in plants genetically engineered by microprojectile bombardment. Mol Biotechnol 6:17–30CrossRefGoogle Scholar
  12. 12.
    Pawlowski WP, Torbert KA, Rines HW, Somers DA (1998) Irregular patterns of transgene silencing in allohexaploid oat. Plant Mol Biol 38:597–607CrossRefGoogle Scholar
  13. 13.
    Morino K, Olsen OA, Shimamoto K (1999) Silencing of an aleurone-specific gene in transgenic rice is caused by a rearranged transgene. Plant J 17:275–285CrossRefGoogle Scholar
  14. 14.
    Tang CM, Sun J, Zhu XF, Guo WZ, Zhang TZ, Shen JL, Gao CF, Zhou WJ, Chen ZX, Guo SD (2000) Inheritance of resistance to Helicoverpa armigera of 3 kinds of transgenic Bt strains available in upland cotton in China. Chin Sci Bull 45:363–367CrossRefGoogle Scholar
  15. 15.
    Zhang B-H, Guo T-L, Wang Q-L (2000) Inheritance and segregation of exogenous genes in transgenic cotton. J Genet 79:71–75CrossRefGoogle Scholar
  16. 16.
    Sachs ES, Bendict JH, Stelly DM, Taylor JF, Altman DW, Berbrich SA, Devis SK (1998) Expression and segregation of gene encoding Bt insecticidal proteins in cotton. Crop Sci 38:1–11CrossRefGoogle Scholar

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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.National Key Laboratory of Crop Genetics and Germplasm EnhancementCotton Research Institute, Nanjing Agricultural UniversityNanjingChina
  2. 2.State Key Laboratory of Crop Genetics & Germplasm Enhancement, College of AgricultureNanjing Agricultural UniversityNanjingChina
  3. 3.Agronomy Department, College of Agriculture and BiotechnologyZhejiang UniversityHangzhouChina
  4. 4.National Key Laboratory of Crop Genetics and Germplasm Enhancement, Cotton Research InstituteNanjing Agricultural UniversityNanjingChina

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