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Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy

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Blood-Brain Barrier

Part of the book series: Neuromethods ((NM,volume 142))

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Abstract

Leukocyte infiltration in the central nervous system (CNS) has been implicated in several neuroinflammatory diseases, being an important step in the development of inflammatory response in the brain. The use of intravital microscopy technique allows direct in vivo assessment of leukocyte rolling and adhesion on cortical meningeal vessels. The current review highlights the use of intravital microscopy and its association with neuroinflammatory parameters in different models of experimental brain diseases such as HSV-1 encephalitis, dengue encephalitis, cerebral malaria, and hepatic encephalopathy. Herein, we also describe a detailed protocol of intravital microscopy of meningeal vessels, its advantages and major concerns.

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Acknowledgments

The authors would like to thank FAPEMIG (Fundação de Amparo à Pesquisa do Estado de Minas Gerais, Brazil), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, Brazil), and CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for financial support. ASM is a 2016 NARSAD Young Investigator Grant Awardee from the Brain and Behavior Research Foundation.

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Authors and Affiliations

  1. Laboratório Interdisciplinar de Investigação Médica, Faculdade de Medicina, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

    Aline Silva de Miranda, Thiago Macedo Cordeiro & Antônio Lúcio Teixeira

  2. Laboratório de Neurobiologia, Departamento de Morfologia, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

    Aline Silva de Miranda

  3. Departamento de Patologia Geral, Universidade Federal de Minas Gerais, Belo Horizonte, Brasil

    Milene Alvarenga Rachid

  4. Neuropsychiatry Program, Department of Psychiatry & Behavioral Sciences, McGovern Medical School, University of Texas Health Science Center at Houston, Houston, TX, USA

    Antônio Lúcio Teixeira

Authors
  1. Aline Silva de Miranda
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  2. Thiago Macedo Cordeiro
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  3. Milene Alvarenga Rachid
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  4. Antônio Lúcio Teixeira
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Editor information

Editors and Affiliations

  1. Department of Psychiatry & Behavioral Sciences, The University of Texas Health Science Center at Houston (UTHealth), Houston, TX, USA

    Tatiana Barichello

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de Miranda, A.S., Cordeiro, T.M., Rachid, M.A., Teixeira, A.L. (2019). Recording Leukocyte Rolling and Adhesion on Meningeal Vessels by Intravital Microscopy. In: Barichello, T. (eds) Blood-Brain Barrier. Neuromethods, vol 142. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8946-1_18

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  • DOI: https://doi.org/10.1007/978-1-4939-8946-1_18

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8945-4

  • Online ISBN: 978-1-4939-8946-1

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