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Procurement, Storage, and Use of Blood in Biobanks

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Biobanking

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1897))

Abstract

Blood is a widely used biospecimen in the field of biobanking, secondary to the ease with which it is collected along with the wide variety of analytes obtained from it for analysis. It carries the potential to further the search for biomarkers in countless diseases; therefore, the standardization and optimization of blood collection procedures is of importance in assuring reproducibility of results. Here, we briefly review procedures for the procurement, storage, and use of blood and its fractions for biobanking purposes. Select commonly used methods for collecting blood with various vacutainer blood collection tubes are described, along with optimal storage conditions of various samples in short- and long-term situations.

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References

  1. Shabihkhani M, Lucey GM, Wei B et al (2014) The procurement, storage, and quality assurance of frozen blood and tissue biospecimens in pathology, biorepository, and biobank settings. Clin Biochem 47(4–5):258–266

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Tuck MK, Chan DW, Chia D et al (2009) Standard operating procedures for serum and plasma collection: early detection research network consensus statement. J Proteome Res 8(1):113–117

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Early Detection Research Network (n.d.) The early detection research network (EDRN) standard operating procedure (SOP) for collection of EDTA plasma. https://edrn.nci.nih.gov/resources/standard-operating-procedures/standard-operating-procedures/

  4. Becton, Dickinson and Company (2010) BD vacutainer venous blood collection tube guide. https://www.bd.com/vacutainer/pdfs/plus_plastic_tubes_wallchart_tubeguide_VS5229.pdf

  5. Vaught JB (2006) Blood collection, shipment, processing, and storage. Cancer Epidemiol Biomarkers Prev. https://doi.org/10.1158/1055-9965.EPI-06-0630

  6. Tolonen H, Kuulasmaa K, Laatikainen T (2002) Chapter 4: Blood; Recommendation for indicators, international collaboration, protocol and manual of operations for chronic disease risk factor surveys; European Health Risk Monitoring (EHRM). http://www.thl.fi/publications/ehrm/product2/part_iii4.htm

  7. Elliott P, Peakman TC (2008) The UK biobank sample handling and storage protocol for the collection, processing and archiving of human blood and urine. Int J Epidemiol 37:234–244

    Article  PubMed  Google Scholar 

  8. Miller SA, Dykes DD, Polesky HF (1988) A simple salting out procedure for extracting DNA from human nucleated cells. Nucleic Acids Res 16(3):1215

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Early Detection Research Network (n.d.) The early detection research network (EDRN) standard operating procedure (SOP) for collection of serum. https://edrn.nci.nih.gov/resources/standard-operating-procedures/standard-operating-procedures/

  10. Teunissen CE, Tumani H, Bennett JL et al (2011) Consensus guidelines for CSF and blood biobanking for CNS biomarker studies. Mult Scler Int 2011:246412

    PubMed  PubMed Central  Google Scholar 

  11. De Paoli P (2005) Biobanking in microbiology: from sample collection to epidemiology, diagnosis and research. FEMS Microbiol Rev 29:897–910

    Article  PubMed  Google Scholar 

  12. Choi EH, Lee SK, Ihm C, Sohn YH (2014) Rapid DNA extraction from dried blood spots on filter paper: potential applications in biobanking. Osong Public Health Res Perspect 5(6):351–357

    Article  PubMed  PubMed Central  Google Scholar 

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Acknowledgments

This work was supported in part by NIH:NCI P50-CA211015, NIH:NIMH U24 MH100929, the Art of the Brain Foundation, and the Henry E. Singleton Brain Cancer Research Program.

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Authors and Affiliations

  1. Division of Neuropathology, Department of Pathology and Laboratory Medicine, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    Jaclyn N. Perry, Afreen Jasim, Amin Hojat & William H. Yong

  2. Brain Tumor Translational Resource, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    William H. Yong

  3. Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    William H. Yong

Authors
  1. Jaclyn N. Perry
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  2. Afreen Jasim
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  3. Amin Hojat
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  4. William H. Yong
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Corresponding author

Correspondence to William H. Yong .

Editor information

Editors and Affiliations

  1. Division of Neuropathology, Department of Pathology and Laboratory Medicine, Brain Tumor Translational Resource, Jonsson Comprehensive Cancer Center, David Geffen School of Medicine at UCLA, Los Angeles, CA, USA

    William H. Yong

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Perry, J.N., Jasim, A., Hojat, A., Yong, W.H. (2019). Procurement, Storage, and Use of Blood in Biobanks. In: Yong, W. (eds) Biobanking. Methods in Molecular Biology, vol 1897. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8935-5_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8935-5_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8933-1

  • Online ISBN: 978-1-4939-8935-5

  • eBook Packages: Springer Protocols

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