Abstract
Inflammasomes are multimeric protein complexes that process IL-1β by cleaving the translated full-length protein into its active IL-1β mature fragment. In oncogene-induced senescence, inflammasomes play a crucial role by regulating IL1R signaling and consequently modulating proliferation and the senescence-associated secretory phenotype (SASP). Inflammasome activation requires two steps: (a) priming of the inflammasome by activation of IL1B expression, followed by (b) cleavage and release of mature IL-1β. In this chapter, we describe methods to detect both stages of inflammasome activation in cellular senescence.
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Fernández-Duran, I., Tarrats, N., Hari, P., Acosta, J.C. (2019). Measuring the Inflammasome in Oncogene-Induced Senescence. In: Demaria, M. (eds) Cellular Senescence. Methods in Molecular Biology, vol 1896. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8931-7_7
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DOI: https://doi.org/10.1007/978-1-4939-8931-7_7
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