Suicide Gene Therapy for Oral Squamous Cell Carcinoma

  • Henrique Faneca
  • Nejat Düzgüneş
  • Maria C. Pedroso de LimaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1895)


Suicide gene therapy has been tested for the treatment of a variety of cancers, including oral cancer. Among the various suicide gene therapy approaches that have been reported, the Herpes Simplex Virus thymidine kinase (HSV-tk)/ganciclovir (GCV) system is one of the most extensively studied systems, holding great promise in cancer therapy. In this chapter, we describe methods to use the HSV-tk/GCV system to achieve antitumor activity, both in cultured oral cancer cells and in orthotopic and subcutaneous murine models of oral squamous cell carcinoma, using ligand-associated lipoplexes for enhancing therapeutic delivery.

Key words

Cationic liposomes Lipoplexes HSV-tk/GCV suicide gene therapy Oral carcinoma Modulation of gap junctions Antitumor activity 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Henrique Faneca
    • 1
  • Nejat Düzgüneş
    • 2
  • Maria C. Pedroso de Lima
    • 1
    Email author
  1. 1.CNC—Center for Neuroscience and Cell BiologyUniversity of CoimbraCoimbraPortugal
  2. 2.Department of Biomedical Sciences, Arthur A. Dugoni School of DentistryUniversity of the PacificSan FranciscoUSA

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