Suicide Gene Therapy of Oral Squamous Cell Carcinoma and Cervical Carcinoma In Vitro
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Suicide gene therapy induced by the Herpes Simplex Virus thymidine kinase/ganciclovir (HSV-tk/GCV) system has been utilized to successfully treat various cancers. We describe TransfeX-mediated transfection of pCMV.Luc into HeLa cervical carcinoma and HSC-3, FaDu, and H357 oral squamous cell carcinoma (OSCC) cell lines in the presence of 10% serum. This method has proved to be highly efficient, with low nonspecific cytotoxicity. The plasmid pNGVL1-tk encoding HSV-tk under the control of the CMV promoter was delivered to the cells in vitro via TransfeX, a cationic liposomal reagent, followed by treatment with ganciclovir. The Alamar Blue cell viability assay was used to determine levels of the suicide effect.
Key wordsHerpes simplex virus thymidine kinase Ganciclovir Oral squamous cell carcinoma Cervical carcinoma Transfection
- 3.Rousseau A, Badoual C (2012) Head and neck: squamous cell carcinoma: an overview. Atlas Genet Cytogenet Oncol Haematol 16:145–155Google Scholar
- 12.Konopka K, Lee A, Moser-Kim N et al (2004) Gene transfer to human oral cancer cells via non-viral vectors and HSV-tk/ganciclovir-mediated cytotoxicity; potential for suicide gene therapy. Gene Ther Mol Biol 8:307–318Google Scholar