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Noninvasive Monitoring of Suicide Gene Therapy by Using Multimodal Molecular Imaging

  • Bryan Holvoet
  • Cindy Leten
  • Christophe M. Deroose
  • Uwe HimmelreichEmail author
Protocol
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Part of the Methods in Molecular Biology book series (MIMB, volume 1895)

Abstract

Cells expressing suicide genes can be used as therapeutic vehicles for difficult-to-treat tumors, for example, if stem cells are used that are able to track infiltrating tumor cells. An alternative application of suicide gene expression is their use as a safety switch in regenerative medicine where the presence of a few pluripotent stem cells could potentially cause unwanted side effects like the formation of teratoma. One potential bottleneck of these applications is that information on the initiation of cell suicide is needed early on, for example, when therapeutic cells have reached infiltrating tumor cells or when teratomas are formed. Therefore, in vivo imaging methods are needed that provide information on target location, (stem) cell location, (stem) cell viability, pathology, and suicide gene expression. This requires multimodal imaging approaches that can provide this information longitudinally and in a noninvasive way. Here, we describe examples of how therapeutic cells can be modified so that they express a suicide gene and genes that can be used for in vivo visualization.

Key words

Suicide gene Magnetic resonance imaging Positron emission tomography Bioluminescence imaging Stem cells 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Bryan Holvoet
    • 1
    • 2
  • Cindy Leten
    • 2
    • 3
  • Christophe M. Deroose
    • 1
    • 2
  • Uwe Himmelreich
    • 2
    • 3
    Email author
  1. 1.Nuclear Medicine and Molecular Imaging, Department of Imaging and PathologyUniversity of LeuvenLeuvenBelgium
  2. 2.Molecular Small Animal Imaging Center (MoSAIC)University of LeuvenLeuvenBelgium
  3. 3.Biomedical MRI Unit, Department of Imaging and PathologyUniversity of LeuvenLeuvenBelgium

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