Metabolic Fingerprinting of Bacteria Exposed to Nanomaterials, Using Online Databases, NMR, and High-Resolution Mass Spectrometry
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Nanomaterials are examined more and more for their antibacterial properties. Herein, we propose a method for assessing the bactericidal properties of nanomaterials against Escherichia coli and Staphylococcus aureus, as well as a method to investigate the metabolic alterations occurring to bacteria, induced by their exposure to nanomaterials. Bacterial metabolome is extracted and metabolic fingerprint is recorded by 1H-NMR. Using metabolomic databases, the tentative metabolites in the samples are revealed, which are further confirmed by UHPLC-HRMS. Finally, conducting a pathway analysis, the metabolic network is revealed.
Key wordsMetabolomics Nanomaterials Bacteria Escherichia coli Staphylococcus aureus Antibacterial properties Metabolite extraction 1H-NMR UHPLC-HRMS Fingerprint
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