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Evaluation of Nrf2 with Exposure to Nanoparticles

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Nanotoxicity

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1894))

Abstract

Transcription factor Nrf2, nuclear factor (erythroid-derived 2)-like 2, is considered a master regulator of redox homeostasis and plays a central role in antioxidant and anti-inflammatory defence. It has been largely reported that oxidative stress is implicated in nanoparticle-induced toxicity with the involvement of Nrf2. Several basic methods for Nrf2 evaluation with exposure to nanoparticles are described in this chapter including real-time reverse transcription-polymerase chain reaction (RT-PCR), western blotting, immunofluorescence staining, electrophoretic mobility shift assay, DNase I footprinting, dimethylsulfate footprinting, protein pulse-chase analysis, and tert-butylhydroquinone treatment.

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Acknowledgments

This work was supported by NSFC grant (No. 81573195, 81172715, and 30800936) and Fujian Provincial Key Laboratory of Environmental Factors and Cancer.

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Authors and Affiliations

  1. Fujian Provincial Key Laboratory of Environmental Factors and Cancer, School of Public Health, Fujian Medical University, Fuzhou, P. R. China

    Fuli Zheng & Huangyuan Li

  2. Department of Preventive Medicine, School of Public Health, Fujian Medical University, Fuzhou, P. R. China

    Fuli Zheng & Huangyuan Li

Authors
  1. Fuli Zheng
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  2. Huangyuan Li
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Editors and Affiliations

  1. Department of Environmental and Occupational Health Sciences, School of Public Health and Information Sciences, University of Louisville, Louisville, KY, USA

    Qunwei Zhang

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Zheng, F., Li, H. (2019). Evaluation of Nrf2 with Exposure to Nanoparticles. In: Zhang, Q. (eds) Nanotoxicity. Methods in Molecular Biology, vol 1894. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8916-4_13

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  • DOI: https://doi.org/10.1007/978-1-4939-8916-4_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8915-7

  • Online ISBN: 978-1-4939-8916-4

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