Method Development of Near-Infrared Spectroscopy Approaches for Nondestructive and Rapid Estimation of Total Protein in Brown Rice Flour

Part of the Methods in Molecular Biology book series (MIMB, volume 1892)


Rice varietal development and improvement programs are constantly seeking means to shorten the breeding cycle in order to deliver new, consumer-acceptable rice varieties to farmers and to consumers. Advances in molecular biology technologies have enabled breeders to use high-throughput genotyping to screen breeding lines. However, current phenotyping technologies, particularly for rice cooking and eating properties, have yet to match the efficiency of genotyping methodologies. A high-throughput and cost-effective phenotyping suite is essential because without phenotype, the value of genotypic information cannot be maximized. In this book chapter, we explore the application of near-infrared spectroscopy (NIRS), a high-throughput and nondestructive approach in characterizing rice grains, primarily describing method development and validation, instrument calibration, upgrading, and maintenance. We then focus on estimating protein content (PC) in brown rice as a case study because (1) PC is an attribute that contributes to the cooking behavior and the eating properties of cooked rice; and (2) proteins contain chemical bonds that can easily be detected by NIRS.

Key words

Protein content Near-infrared spectroscopy (NIRS) 



The authors thank the following GQNSL staff for the technical assistance in generating the protein data: Jose Rosales, Edgar Amoloza, Leonel Borebor, Anna Carissa Basilio, and Ruben Chavez. This work has been supported under the CGIAR thematic area Global Rice Agri-Food System CRP, RICE, Stress-Tolerant Rice for Africa and South Asia (STRASA) Phase III, and Australian Centre for International Agricultural Research (Project ID CIM/2016/046) funding.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.International Rice Research InstituteLos BañosPhilippines

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