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Rice Grain Quality pp 187–199Cite as

Rice Grain Quality Benchmarking Through Profiling of Volatiles and Metabolites in Grains Using Gas Chromatography Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1892))

Abstract

Gas chromatograph coupled with mass spectrometer is widely used to profile volatiles and metabolites from the homogenized rice flour obtained from mature grains. Rice grains consist of central endosperm which stores majorly starch and, in addition, accumulate various storage proteins as storage reserves. The outer nutritious aleurone layer stores lipids, sugar alcohols, volatiles, antioxidants, vitamins, and various micronutrients. Once paddy sample is dehulled, milled, and ground cryogenically, the brown rice flour is subjected to extraction of primary metabolites and volatiles using an appropriate extraction method. In metabolite profiling of the liquid extract obtained from the rice sample, mixture is initially subjected to methoxyamination then silylation before being subjected to untargeted metabolite profiling. Peaks obtained are processed for noise reduction and specific signal selection. Volatile compounds are initially extracted using a solid phase adsorbent prior to analysis. All these compounds, metabolites, and volatiles are detected in the mass selective detector by fragmentation at 70 eV ionization energy and the resultant mass spectrum compared with a built-in library of compounds. Data mined from the gas chromatography mass spectrometry analysis are then subjected to post-processing statistical analysis.

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Acknowledgments

This work has been supported under the CGIAR thematic area Global Rice Agri-Food System CRP, RICE, Stress-Tolerant Rice for Africa and South Asia (STRASA) Phase III funding.

Author information

Authors and Affiliations

  1. International Rice Research Institute, Los Baños, Laguna, Philippines

    Cindy Llorente, Rosario Jimenez & Nese Sreenivasulu

  2. Shimadzu (Asia Pacific) Pte. Ltd., Singapore, Singapore

    Jackie

  3. Max-Planck-Institute of Molecular Plant Physiology, Potsdam-Golm, Germany

    Yariv Brotman & Alisdair R. Fernie

  4. Department of Life Sciences, Ben-Gurion University of the Negev, Beersheba, Israel

    Yariv Brotman

  5. Center of Plant System Biology and Biotechnology, Plovdiv, Bulgaria

    Alisdair R. Fernie

Authors
  1. Cindy Llorente
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  2. Rosario Jimenez
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  3. Jackie
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  4. Yariv Brotman
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  5. Alisdair R. Fernie
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  6. Nese Sreenivasulu
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Corresponding author

Correspondence to Nese Sreenivasulu .

Editor information

Editors and Affiliations

  1. Grain Quality and Nutrition Center, International Rice Research Institute, Manila, Philippines

    Nese Sreenivasulu

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Llorente, C., Jimenez, R., Jackie, Brotman, Y., Fernie, A.R., Sreenivasulu, N. (2019). Rice Grain Quality Benchmarking Through Profiling of Volatiles and Metabolites in Grains Using Gas Chromatography Mass Spectrometry. In: Sreenivasulu, N. (eds) Rice Grain Quality. Methods in Molecular Biology, vol 1892. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8914-0_11

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  • DOI: https://doi.org/10.1007/978-1-4939-8914-0_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8912-6

  • Online ISBN: 978-1-4939-8914-0

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