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Hippo Pathway Regulation by Tyrosine Kinases

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The Hippo Pathway

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1893))

Abstract

The Hippo pathway utilizes a well-characterized Ser/Thr kinase cascade to control the downstream effectors, Yap and Taz. In addition, Yap/Taz and other Hippo pathway components are directly regulated by tyrosine kinases (TKs). The methodological strategies described here use the example of the c-Abl non-receptor TK and the Yap substrate to outline the steps used to identify and to validate tyrosine phosphorylation sites, including bioinformatic approaches, ectopic expression of proteins in transfected tissue culture cells, and mutagenesis of endogenous proteins by CRISPR-Cas9. These general strategies can be applied to investigate regulation of protein signaling moieties by tyrosine phosphorylation in the context of distinct TKs.

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Authors and Affiliations

  1. Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel

    Nina Reuven, Matan Shanzer & Yosef Shaul

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  1. Nina Reuven
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  2. Matan Shanzer
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  3. Yosef Shaul
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Correspondence to Yosef Shaul .

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  1. Cancer Institute, University College London, London, UK

    Alexander Hergovich

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Reuven, N., Shanzer, M., Shaul, Y. (2019). Hippo Pathway Regulation by Tyrosine Kinases. In: Hergovich, A. (eds) The Hippo Pathway. Methods in Molecular Biology, vol 1893. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8910-2_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8910-2_17

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