Abstract
Lactic acid bacteria (LAB) can improve human intraintestinal conditions. One reason is that ingestion of LAB prevents bacterial diarrhea. Furthermore, inflammation of human intestines can be caused by a lipopolysaccharide (LPS) component in the cell walls of gram-negative bacteria. This chapter describes a method of LPS elimination using lactic acid bacteria (LAB). First, the LPS concentration is assayed using an LPS assay kit with the limulus cascade reaction made by limulus amebocyte lysate. Some LABs, four bacillus strains and one coccus strain, have LPS-elimination activity. Particularly, the coccus strain Pediococcus pentosaceus eliminates LPS to 43%. The cells fractionate and eliminate four fractions: the extracellular fraction, cell membrane fraction, cytoplasm fraction, and cell wall fraction. Only the cell wall digesting fraction eliminates LPS to 45%. Results confirm that the LAB eliminates all LPS having O-antigen under a low-sugar medium condition at temperatures of 15–30 °C. This method can be used for assay of LPS elimination by LABs exactly and easily for the probiotics field.
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References
Metchnikoff É (1908) The Prolongation of Life. In: Mitchell PC (trans., ed) Optimistic Studies. GP Putnam's, New York
Gorbach SL (1990) Lactic acid bacteria and human health. Annal Med 22:37–41
Caradonna L, Amati L, Magrone T et al (2000) Enteric bacteria, lipopolysaccharides and related cytokines in inflammatory bowel disease: biological and clinical significance. J Endotoxin Res 6:205–214
Van Deventer SJ, Buller HR, ten Cate JW et al (1990) Experimental endotoxemia in humans: analysis of cytokine release and coagulation, fibrinolytic, and complement pathways. Blood 76:2520–2526
Morrison DC, Jacobs DM (1976) Binding of polymyxin B to the lipid a portion of bacterial lipopolysaccharides. Immunochemistry 13:813–818
Van Amersfoort ES, Van Berkel TJC, Kuiper J (2003) Receptors, mediators, and mechanisms involved in bacterial Sepsis and septic shock. Clin Microbiol Rev 16:379–414
Ishikawa H, Akedo I, Umesaki Y et al (2003) Randomized controlled trial of the effect of bifidobacteria-fermented milk on ulcerative colitis. J Am Coll Nutr 22:56–63
Kondo A, Asami K, Suda Y et al (2016) Isolation of endotoxin eliminating lactic acid Bacteria and a property of endotoxin eliminating protein. J Food Sci 81:1457–1465
Urich K (2013) Comparative animal biochemistry pp 206–207, Springer Science & Business Media. Germany, Berlin
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Kanauchi, M., Kondo, A., Asami, K. (2019). Eliminating Lipopolysaccharide (LPS) Using Lactic Acid Bacteria (LAB) and a Fraction of its LPS-Elimination Protein. In: Kanauchi, M. (eds) Lactic Acid Bacteria. Methods in Molecular Biology, vol 1887. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8907-2_15
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DOI: https://doi.org/10.1007/978-1-4939-8907-2_15
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Publisher Name: Humana Press, New York, NY
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