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Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

  • Alba Orea-Soufi
  • David Dávila
  • María Salazar-Roa
  • María de Mar Lorente
  • Guillermo VelascoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1890)

Abstract

Phosphorylation of FOXO transcription factors is one of the key mechanisms involved in the regulation of the activity, nucleo-cytosolic shuttling, and stability of this family of proteins. Here, we describe several experimental approaches allowing analysis of changes in the phosphorylation of these proteins upon exposure to different stimuli.

Key words

FOXO phosphorylation Western blot Immunofluorescence cell fractioning 

Notes

Acknowledgments

Work in G Velasco’s group is funded by the PI15/00339 grant, integrated into the State Plan for R & D + I2013-2016 and funded by the Instituto de salud Carlos III (ISCIII) and the European Regional Development Fund (ERDF), by the GA 721532 Marie Sklowdoska-Curie Innovative Training Network (ITN) action TRAIN (Tribbles research and Innovation Network) funded by the H2020 program of the European Commission and by a grant from Voices Against Brain Cancer. We than all member of the L1-L2 groups for their contribution to the setup of the experimental approaches described in this chapter.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Alba Orea-Soufi
    • 1
    • 2
  • David Dávila
    • 1
    • 2
  • María Salazar-Roa
    • 1
    • 2
  • María de Mar Lorente
    • 1
    • 2
  • Guillermo Velasco
    • 1
    • 2
    Email author
  1. 1.Department of Biochemistry and Molecular Biology, School of BiologyComplutense UniversityMadridSpain
  2. 2.Instituto de Investigaciones Sanitarias San Carlos (IdISSC)MadridSpain

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