Following Transcriptome to Uncover FOXO Biological Functions
Two and half million red blood cells (RBC) are generated every second in a healthy adult. The process of RBC production known as erythropoiesis requires a meticulous synchrony between signaling processes and the activity of many transcription factor complexes. FOXO3 is a transcription factor that is responsive to signaling processes and essential for the erythroid proliferation and maturation, RBC formation, and lifespan. Here, we discuss how using an integrated computational and experimental systems biology approach new and unanticipated FOXO3 functions in terminal erythropoiesis were uncovered. These combinatory approaches identified FOXO3 as a key regulator of terminal erythropoiesis. As a result, a new mode of FOXO3 participation in erythroid transcription complex formation has been proposed.
Key wordsErythropoiesis Red blood cell (RBC) Enucleation Mitochondria Mitophagy FOXO FOXO3 Transcriptome RNA sequencing
The work in the Ghaffari lab is supported by grants from National Institutes of Health (NCI and NHLBI). Raymond Liang is supported by a pre-doctoral fellowship from the American Heart Association.
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