Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO
Cancer cells are known to inactivate tumor suppressor proteins by triggering their anomalous subcellular location. It has been well established that the aberrant location of FOXO proteins is linked to tumor formation, progression of the same, or resistance to anti-neoplastic treatment. Furthermore, the abnormal location of FOXO has also been considered a potential biomarker for diabetic complications or longevity in different organisms. Here, we describe the immunodetection of endogenous FOXO by confocal microscopy, which can be used as a chemical tool to quantify FOXO expression levels, its cellular location, and even its active/inactive forms with relevant antibodies.
Key wordsBiomarker FOXO proteins Nuclear translocation Immunodetection Confocal microscopy
- 4.Hofman FM, Taylor CR (2001) Immunohistochemistry. Curr Protoc Immunol. https://doi.org/10.1002/0471142735.im2104s103
- 19.Terragni J, Graham JR, Adams KW et al (2008) Phosphatidylinositol 3-kinase signaling in proliferating cells maintains an anti-apoptotic transcriptional program mediated by inhibition of FOXO and non-canonical activation of NFκB transcription factors. BMC Cell Biol 9:6. https://doi.org/10.1186/1471-2121-9-6 CrossRefPubMedPubMedCentralGoogle Scholar