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Myogenesis pp 245–254Cite as

Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1889))

Abstract

This chapter describes a protocol for the isolation of larval zebrafish muscle stem/progenitor cells by fluorescence-activated cell sorting (FACS). This method has been successfully applied to isolate pax3a expressing cells 3 days following needle stab skeletal muscle injury. The cell sorting strategy described here can easily be adapted to any cell type at embryonic or larval stages. RNA extracted from the sorted cells can be used for subsequent downstream applications such as quantitative PCR (qPCR), microarrays, or next generation sequencing.

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Author information

Authors and Affiliations

  1. Australian Regenerative Medicine Institute, Monash University, Clayton, VIC, Australia

    Dhanushika Ratnayake & Peter D. Currie

  2. European Molecular Biology Laboratory Australia Melbourne Node, Monash University, Clayton, VIC, Australia

    Dhanushika Ratnayake & Peter D. Currie

Authors
  1. Dhanushika Ratnayake
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  2. Peter D. Currie
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Corresponding author

Correspondence to Dhanushika Ratnayake .

Editor information

Editors and Affiliations

  1. Nofima AS, Ås, Norway

    Sissel Beate Rønning

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Ratnayake, D., Currie, P.D. (2019). Fluorescence-Activated Cell Sorting of Larval Zebrafish Muscle Stem/Progenitor Cells Following Skeletal Muscle Injury. In: Rønning, S. (eds) Myogenesis. Methods in Molecular Biology, vol 1889. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8897-6_14

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  • DOI: https://doi.org/10.1007/978-1-4939-8897-6_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8896-9

  • Online ISBN: 978-1-4939-8897-6

  • eBook Packages: Springer Protocols

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