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Atomic Force Microscopy pp 45–59Cite as

Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy

Part of the Methods in Molecular Biology book series (MIMB,volume 1886)

Abstract

Supported lipid bilayers represent a very attractive way to mimic biological membranes, especially to investigate molecular mechanisms associated with the lateral segregation of membrane components. Observation of these model membranes with high-speed atomic force microscopy (HS-AFM) allows the capture of both topography and dynamics of membrane components, with a spatial resolution in the nanometer range and image capture time of less than 1 s. In this context, we have developed new protocols adapted for HS-AFM to form supported lipid bilayers on small mica disks using the vesicle fusion or Langmuir-Blodgett methods. In this chapter we describe in detail the protocols to fabricate supported artificial bilayers as well as the main guidelines for HS-AFM imaging of such samples.

Key words

  • Atomic force microscopy
  • Lipid
  • Artificial membrane
  • Supported lipid bilayer
  • Vesicle fusion
  • Langmuir
  • Dynamics

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  • DOI: 10.1007/978-1-4939-8894-5_3
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Acknowledgments

The research has been supported by CNRS (PEM and EL), INSERM (PEM), Institut Carnot (EL), and by the ANR program (ANR-11-nano-009-04, ANR-08-NANO-010-03, ANR- 08-PCVI-0003-02, the EpiGenMed Labex ANR-10-LABX-12-01 and the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05). We are grateful to our collaborators involved in the project, P. Dosset, J. Kokavecz, and C. Le Grimellec.

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Authors and Affiliations

  1. INSERM, U1054, Montpellier, France

    Hussein Nasrallah, Anthony Vial, Jérémy Soulier, Luca Costa, Cédric Godefroy & Pierre-Emmanuel Milhiet

  2. Centre de Biochimie Structurale, Université de Montpellier, CNRS, UMR 5048, Montpellier, France

    Hussein Nasrallah, Anthony Vial, Jérémy Soulier, Luca Costa, Cédric Godefroy & Pierre-Emmanuel Milhiet

  3. ICB UMR CNRS 6303, University of Bourgogne Franche-Comte, Dijon, France

    Nicolas Pocholle, Eric Bourillot & Eric Lesniewska

Authors
  1. Hussein Nasrallah
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  2. Anthony Vial
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  3. Nicolas Pocholle
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  4. Jérémy Soulier
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  5. Luca Costa
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  6. Cédric Godefroy
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  7. Eric Bourillot
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  8. Eric Lesniewska
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  9. Pierre-Emmanuel Milhiet
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Corresponding author

Correspondence to Pierre-Emmanuel Milhiet .

Editor information

Editors and Affiliations

  1. Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

    Nuno C. Santos

  2. Instituto de Medicina Molecular, Faculdade de Medicina, Universidade de Lisboa, Lisbon, Portugal

    Filomena A. Carvalho

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Nasrallah, H. et al. (2019). Imaging Artificial Membranes Using High-Speed Atomic Force Microscopy. In: Santos, N., Carvalho, F. (eds) Atomic Force Microscopy. Methods in Molecular Biology, vol 1886. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8894-5_3

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  • DOI: https://doi.org/10.1007/978-1-4939-8894-5_3

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8893-8

  • Online ISBN: 978-1-4939-8894-5

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