Antimicrobial Peptides: Effect on Bacterial Cells

  • Marco M. DominguesEmail author
  • Mário R. Felício
  • Sónia GonçalvesEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1886)


Antimicrobial peptides (AMPs) are one of the most promising alternatives to conventional antibiotics. Atomic force microscopy (AFM), as imaging and force spectroscopy tool, has been applied to study their mechanism of action and development. Here, we describe different methods to be applied in the study of AMP effects on bacteria, either by imaging or by force spectroscopy studies, essential to underlie their action and to identify possibly outcomes of the same.

Key words

Antimicrobial peptides Atomic force microscopy Gram-negative bacteria Gram-positive bacteria Imaging Force spectroscopy 



This work was supported by Fundação para a Ciência e a Tecnologia – Ministério da Ciência, Tecnologia e Ensino Superior (FCT-MCTES, Portugal), including FCT-MCTES fellowships SFRH/BPD/122779/2016 and SPRH/BD/100517/2014 to MMD and MRF, respectively.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Instituto de Medicina Molecular, Faculdade de MedicinaUniversidade de LisboaLisbonPortugal

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