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Prenatal Diagnosis pp 297–307Cite as

Quad Screen Test, A Multiplexed Biomarker Assay for Prenatal Screening to Assess Birth Defects: The Columbia University Experience Using the Beckman Access2 Immunoassay Analyzer and Benetech PRA

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1885))

Abstract

In the prenatal quad screen, the levels of four analytes in maternal serum are used to calculate the risk of serious birth defects. The Beckman Access2 Immunoassay System is an automated analyzer that enables rapid measurement of alpha-fetoprotein, unconjugated estriol, human chorionic gonadotropin, and dimeric inhibin A. The Benetech PRA software package is used to convert maternal serum analyte concentrations to multiples of the median (MoM) and calculates the risks of particular birth defects. The results from this simple and minimally invasive screen determine the need for more sensitive, specific, and usually riskier diagnostic procedures. We present herein some recent data from our experience at Columbia University Medical Center in New York, NY, using the Beckman Access2 immunoassay analyzer and Benetech PRA software package.

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Author information

Author notes

  1. Awet Tecleab and Alex K. Lyashchenko contributed equally to this work.

Authors and Affiliations

  1. Department of Pathology and Laboratory Medicine, Staten Island University Hospital, Staten Island, NY, USA

    Awet Tecleab

  2. Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA

    Alex K. Lyashchenko & Alex J. Rai

Authors
  1. Awet Tecleab
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  2. Alex K. Lyashchenko
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  3. Alex J. Rai
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Corresponding author

Correspondence to Alex J. Rai .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA

    Brynn Levy

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Tecleab, A., Lyashchenko, A.K., Rai, A.J. (2019). Quad Screen Test, A Multiplexed Biomarker Assay for Prenatal Screening to Assess Birth Defects: The Columbia University Experience Using the Beckman Access2 Immunoassay Analyzer and Benetech PRA. In: Levy, B. (eds) Prenatal Diagnosis. Methods in Molecular Biology, vol 1885. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8889-1_20

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  • DOI: https://doi.org/10.1007/978-1-4939-8889-1_20

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8887-7

  • Online ISBN: 978-1-4939-8889-1

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