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Next Generation Sequencing of Prenatal Structural Chromosomal Rearrangements Using Large-Insert Libraries

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Prenatal Diagnosis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1885))

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Abstract

Precise tests for genomic structural variation (SV) are essential for accurate diagnosis of prenatal genome abnormalities. The two most ubiquitous traditional methods for prenatal SV assessment, karyotyping and chromosomal microarrays, do not provide sufficient resolution for some clinically actionable SVs. Standard whole-genome sequencing (WGS) overcomes shortcomings of traditional techniques by providing base-pair resolution of the entire accessible genome. However, while sequencing costs have continued to decline in recent years, conventional WGS costs remain high for most routine clinical applications. Here, we describe a specialized WGS technique using large inserts (liWGS; also known as “jumping libraries”) to resolve large (>5000–10,000 nucleotides) SVs at kilobase-resolution in prenatal samples, and at a fraction of the cost of standard WGS. We explicate the protocols for generating liWGS libraries and supplement with an overview for processing and analyzing liWGS data.

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Authors and Affiliations

  1. Massachusetts General Hospital, Boston, MA, USA

    Benjamin B. Currall, Caroline W. Antolik, Ryan L. Collins & Michael E. Talkowski

  2. Broad Institute, Harvard Medical School, Cambridge, MA, USA

    Benjamin B. Currall, Caroline W. Antolik, Ryan L. Collins & Michael E. Talkowski

Authors
  1. Benjamin B. Currall
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  2. Caroline W. Antolik
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  3. Ryan L. Collins
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  4. Michael E. Talkowski
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Corresponding author

Correspondence to Michael E. Talkowski .

Editor information

Editors and Affiliations

  1. Department of Pathology and Cell Biology, Vagelos College of Physicians and Surgeons, Columbia University Irving Medical Center, New York, NY, USA

    Brynn Levy

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Currall, B.B., Antolik, C.W., Collins, R.L., Talkowski, M.E. (2019). Next Generation Sequencing of Prenatal Structural Chromosomal Rearrangements Using Large-Insert Libraries. In: Levy, B. (eds) Prenatal Diagnosis. Methods in Molecular Biology, vol 1885. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8889-1_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8889-1_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8887-7

  • Online ISBN: 978-1-4939-8889-1

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