Abstract
Droplet digital PCR (ddPCR) allows precise quantitation of individual copies of DNA. Using allele-specific fluorescent oligonucleotide probes, ddPCR can enumerate both mutant and wild-type alleles enabling highly sensitive detection and quantitation of the variant allele frequency of mutated genes. In this protocol, we describe a method for using ddPCR to detect mutations in genomic DNA with a sensitivity of up to 1 in 50,000 DNA copies.
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Parkin, B. (2019). Rare Variant Quantitation Using Droplet Digital PCR. In: Malek, S. (eds) Chronic Lymphocytic Leukemia. Methods in Molecular Biology, vol 1881. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8876-1_18
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DOI: https://doi.org/10.1007/978-1-4939-8876-1_18
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8875-4
Online ISBN: 978-1-4939-8876-1
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