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Microinjection of Marine Fish Eggs

Part of the Methods in Molecular Biology book series (MIMB,volume 1874)

Abstract

Microinjection is a powerful tool for studying embryonic development and analyzing gene functions in fish. This technique was first applied to model species of fish such as zebrafish and medaka whose egg chorions could be removed or softened before microinjection. Recent progress in genome editing using TALEN and CRISPR has opened the opportunity to analyze gene functions in a much wider range of fish including those important to marine aquaculture. Therefore, application of the microinjection technique is also required in these species. However, the characteristics of fish eggs vary widely between species and several technical difficulties need to be overcome in order to use microinjection in a wider range of species. To obtain consistent results with microinjection, an optimal method has to be developed for each target species. In this chapter, we describe the physical characteristics of the eggs of fish species that have been used in microinjection experiments in our laboratory and detail the microinjection system we developed for fish eggs with a hard chorion, such as those of marine species.

Key words

  • Microinjection
  • Fish egg
  • Marine fish species
  • Glass needle
  • Yolk
  • Blastodisc
  • Aquaculture species

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  • DOI: 10.1007/978-1-4939-8831-0_27
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Correspondence to Rie Goto .

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Goto, R., Saito, T., Matsubara, T., Yamaha, E. (2019). Microinjection of Marine Fish Eggs. In: Liu, C., Du, Y. (eds) Microinjection. Methods in Molecular Biology, vol 1874. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8831-0_27

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  • DOI: https://doi.org/10.1007/978-1-4939-8831-0_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8830-3

  • Online ISBN: 978-1-4939-8831-0

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