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Microinjection pp 475-487 | Cite as

Microinjection of Marine Fish Eggs

  • Rie GotoEmail author
  • Taiju Saito
  • Takahiro Matsubara
  • Etsuro Yamaha
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1874)

Abstract

Microinjection is a powerful tool for studying embryonic development and analyzing gene functions in fish. This technique was first applied to model species of fish such as zebrafish and medaka whose egg chorions could be removed or softened before microinjection. Recent progress in genome editing using TALEN and CRISPR has opened the opportunity to analyze gene functions in a much wider range of fish including those important to marine aquaculture. Therefore, application of the microinjection technique is also required in these species. However, the characteristics of fish eggs vary widely between species and several technical difficulties need to be overcome in order to use microinjection in a wider range of species. To obtain consistent results with microinjection, an optimal method has to be developed for each target species. In this chapter, we describe the physical characteristics of the eggs of fish species that have been used in microinjection experiments in our laboratory and detail the microinjection system we developed for fish eggs with a hard chorion, such as those of marine species.

Key words

Microinjection Fish egg Marine fish species Glass needle Yolk Blastodisc Aquaculture species 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Rie Goto
    • 1
    Email author
  • Taiju Saito
    • 1
  • Takahiro Matsubara
    • 1
  • Etsuro Yamaha
    • 2
  1. 1.South Ehime Fisheries Research CenterEhime UniversityMinamiuwagunJapan
  2. 2.Nanae Fresh Water Laboratory, Field Science Center for Northern BiosphereHokkaido UniversityKamedaJapan

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