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Microinjection in Zebrafish for Genome Editing and Functional Studies

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Microinjection

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1874))

Abstract

A major strength of zebrafish as a model organism is their rapid, in vitro development. The easy access to embryos compared to mammals, allows larval molecular and cellular composition to be manipulated by microinjection, providing a powerful avenue for biological and translational studies. Here, we describe the essential steps and different applications of microinjection in zebrafish for genome editing and functional studies, along with some experimental tips that are critical for microinjection success.

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Acknowledgments

This work was supported by the Intramural Research Program of the National Human Genome Research Institute (ZIAHG200386-05). We thank Dr. Benjamin Feldman from NICHD for technical advice on microinjections; Dr. Gaurav K. Varshney and other members of the Burgess laboratory and NHGRI Zebrafish Core for improving the protocols.

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Authors and Affiliations

  1. Translational and Functional Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA

    Wuhong Pei & Shawn M. Burgess

Authors
  1. Wuhong Pei
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  2. Shawn M. Burgess
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Corresponding author

Correspondence to Shawn M. Burgess .

Editor information

Editors and Affiliations

  1. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Chengyu Liu

  2. National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA

    Yubin Du

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Pei, W., Burgess, S.M. (2019). Microinjection in Zebrafish for Genome Editing and Functional Studies. In: Liu, C., Du, Y. (eds) Microinjection. Methods in Molecular Biology, vol 1874. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8831-0_26

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  • DOI: https://doi.org/10.1007/978-1-4939-8831-0_26

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8830-3

  • Online ISBN: 978-1-4939-8831-0

  • eBook Packages: Springer Protocols

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