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Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS

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LC-MS in Drug Analysis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1872))

Abstract

High-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) has become a primary analytical methodology in therapeutic drug monitoring of antiepileptic drugs (AEDs). To demonstrate the utility of LC-MS/MS in measuring drug concentrations in serum or plasma, analysis of lacosamide (Vimpat™) is discussed in this chapter. Lacosamide is an example of the newer-generation AEDs. The drug is extracted by protein precipitation and dilution of the serum specimen. A small volume of the extracted specimen is injected into a reversed-phase chromatography column, and lacosamide is identified by positive electrospray ionization (ESI) mass spectrometry in the multiple reaction monitoring (MRM) mode, which provides selectivity for quantitative analysis. A deuterated internal standard is used to correct for any loss of analyte during the process of extraction and analysis. A seven-point calibration curve and two levels of quality controls are included in each batch.

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Author information

Authors and Affiliations

  1. Quest Diagnostics Nichols Institute of Valencia, Valencia, CA, USA

    He Sarina Yang

  2. Quest Diagnostics Nichols Institute of Chantilly, Chantilly, VA, USA

    Leslie Edinboro

Authors
  1. He Sarina Yang
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  2. Leslie Edinboro
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Corresponding author

Correspondence to He Sarina Yang .

Editor information

Editors and Affiliations

  1. Department of Laboratory, Medicine and Pathology, Mayo Clinic, Rochester, MN, USA

    Loralie J. Langman

  2. Department of Laboratory, Medicine and Pathology, Mayo Clinic in Arizona, Scottsdale, AZ, USA

    Christine L.H. Snozek

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Yang, H.S., Edinboro, L. (2019). Therapeutic Drug Monitoring of Lacosamide by LC-MS/MS. In: Langman, L., Snozek, C. (eds) LC-MS in Drug Analysis. Methods in Molecular Biology, vol 1872. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8823-5_7

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  • DOI: https://doi.org/10.1007/978-1-4939-8823-5_7

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8822-8

  • Online ISBN: 978-1-4939-8823-5

  • eBook Packages: Springer Protocols

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