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Targeted Proteomics

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Book cover Functional Proteomics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1871))

Abstract

Targeted proteomics detects proteins of interest with high sensitivity, quantitative accuracy, and reproducibility. In a targeted proteomics assay, surrogate peptides are generated by proteolytic digestion of target proteins and selected reaction monitoring (SRM) assays are developed to quantify these peptides using liquid chromatography-tandem mass spectrometry (LC-MS/MS). In this report, we describe the details of quantitative analysis of target protein in cells and tissue samples.

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Author information

Authors and Affiliations

  1. School of Pharmacy, Nanjing Medical University, Nanjing, China

    Yun Chen & Liang Liu

Authors
  1. Yun Chen
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  2. Liang Liu
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Corresponding author

Correspondence to Yun Chen .

Editor information

Editors and Affiliations

  1. Array Bridge Inc., St. Louis, MO, USA

    Xing Wang

  2. Biotech Support Group LLC, Monmouth Junction, NJ, USA

    Matthew Kuruc

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Chen, Y., Liu, L. (2019). Targeted Proteomics. In: Wang, X., Kuruc, M. (eds) Functional Proteomics. Methods in Molecular Biology, vol 1871. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8814-3_17

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  • DOI: https://doi.org/10.1007/978-1-4939-8814-3_17

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8813-6

  • Online ISBN: 978-1-4939-8814-3

  • eBook Packages: Springer Protocols

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