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Flow Cytometric Analysis of Brain Tumor Stem Cells

Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1869)

Abstract

As a useful biotechnology, flow cytometry has revolutionized the field of cell analysis through its dynamic system that employs fluidics, optics, and electronics. It was first used to analyze DNA, but is often used to determine biomarker expression, as well as to characterize and sort cells, in accordance with various parameters. A common application of flow cytometry is the identification and isolation of a distinct cancer cell population, known as cancer stem cells (CSCs). Various biomarkers have been used to elucidate this proportion of cells within the brain, termed brain tumor initiating cells (BTICs). Here, we discuss methodology to prepare BTICs for flow cytometric analysis that includes the expression of markers.

Key words

Flow cytometry Cell sorting Cell analysis biomarkers 
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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.McMaster Stem Cell and Cancer Research InstituteMcMaster UniversityHamiltonCanada
  2. 2.Department of Biochemistry and Biomedical Sciences, Faculty of Health SciencesMcMaster UniversityHamiltonCanada
  3. 3.Department of Surgery, Faculty of Health SciencesMcMaster UniversityHamiltonCanada
  4. 4.Stem Cell and Cancer Research InstituteMcMaster UniversityHamiltonCanada
  5. 5.Department of Biochemistry and Biomedical SciencesMcMaster UniversityHamiltonCanada
  6. 6.Department of SurgeryMcMaster UniversityHamiltonCanada

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