Abstract
Since mice are widely used to establish rheumatoid arthritis models, assessment of the pathogenesis of local arthritis is fundamental. Proteins are the most diverse group of biologically important molecules and are essential for cellular structure and function. The first step in pathogenesis-related protein analysis is joint tissue extraction. Unlike other large rodents, obtaining synovium from model mice is challenging, since it is so small and fragile. In this chapter, methods for harvesting synovium through a quadriceps approach and preparing protein extracts are introduced.
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Liu, S., Takemasa, E. (2018). Preparation of Joint Extracts. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 1868. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8802-0_6
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DOI: https://doi.org/10.1007/978-1-4939-8802-0_6
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8801-3
Online ISBN: 978-1-4939-8802-0
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