Abstract
Nucleic acid aptamers are therapeutic agents consisting of short single-strand DNA or RNA oligonucleotides, which have the ability to bind to target therapeutic molecules with high affinity and specificity, and have been developed as potent drugs for the treatment of rheumatoid arthritis. Aptamers have unique and advantageous features over antibodies, such as superior affinity with nano- or pico-molar dissociation constants, and ease of chemical synthesis, modification, and inactivation by designing antisense sequences. In this chapter, using a DNA-oligonucleotide pool, the technology of proteoliposome-systematic evolution of ligands by exponential enrichment (SELEX) is introduced. By using this technique, potential therapeutic agents with high affinity and specificity could be obtained.
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Liu, S., Suzuki, Y., Inui, M. (2018). Generation of Specific Aptamers. In: Liu, S. (eds) Rheumatoid Arthritis. Methods in Molecular Biology, vol 1868. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8802-0_11
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DOI: https://doi.org/10.1007/978-1-4939-8802-0_11
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8801-3
Online ISBN: 978-1-4939-8802-0
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