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Electrophoretic Separation of Proteins pp 315–326Cite as

Characterization of New Cyclic d,l-α-Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1855))

Abstract

The self-assembly of peptide nanotubes (PNTs) depends on the structure and chemistry of cyclic peptide (CP) monomers, impacting on their properties, which makes the choice of their monomers and their characterization a high challenge. For this purpose, we developed for the first time a capillary electrophoresis coupled to electrospray ionization mass spectrometry (CE-ESI-MS) methodology and characterized a set of eight original CP sequences of 8, 10, and 12 d,l-α-alternate amino acids with a controlled internal diameter (from 7 to 13 Å) and various properties (diameter, global surface charge, hydrophobicity). This new CE-ESI-MS methodology allows verifying the structure, the purity, as well as the stability (when stored during several months) of interesting potential precursors for PNTs that could be employed as nanoplatforms in diagnostics or pseudo sieving tools for separation purposes.

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Author information

Authors and Affiliations

  1. Chimie ParisTech PSL, Ecole Nationale Supérieure de Chimie, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France

    María Dámaris Cortez-Díaz, Fanny d’Orlyé & Anne Varenne

  2. CNRS, Unité de Technologies Chimiques et Biologiques pour la Santé UMR 8258, Paris, France

    María Dámaris Cortez-Díaz, Fanny d’Orlyé & Anne Varenne

  3. Université Paris Descartes, Unité de Technologies Chimiques et Biologiques pour la Santé, Paris, France

    María Dámaris Cortez-Díaz, Fanny d’Orlyé & Anne Varenne

  4. INSERM, Unité de Technologies Chimiques et Biologiques pour la Santé (N°1022), Paris, France

    María Dámaris Cortez-Díaz, Fanny d’Orlyé & Anne Varenne

  5. Departamento de Quimica, Universidad de Guanajuato, Guanajuato, Mexico

    María Dámaris Cortez-Díaz

Authors
  1. María Dámaris Cortez-Díaz
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  2. Fanny d’Orlyé
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  3. Anne Varenne
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Corresponding author

Correspondence to Anne Varenne .

Editor information

Editors and Affiliations

  1. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    Biji T. Kurien

  2. Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA

    R. Hal Scofield

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Cortez-Díaz, M.D., d’Orlyé, F., Varenne, A. (2019). Characterization of New Cyclic d,l-α-Alternate Amino Acid Peptides by Capillary Electrophoresis Coupled to Electrospray Ionization Mass Spectrometry. In: Kurien, B., Scofield, R. (eds) Electrophoretic Separation of Proteins. Methods in Molecular Biology, vol 1855. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8793-1_27

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  • DOI: https://doi.org/10.1007/978-1-4939-8793-1_27

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8792-4

  • Online ISBN: 978-1-4939-8793-1

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