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Identification of Proteins on Archived 2D Gels

  • Hiroyuki Matsumoto
  • Nobuaki Takemori
  • Naoka Komori
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1855)

Abstract

Separation technology of proteins from a complex mixture by two-dimensional gel (2D gel) was invented more than 40 years ago. With a good laboratory practice, the 2D gels are likely to be dried and stored at ambient temperature as archived record. Up until the beginning of this century, it had been difficult to identify the protein spots isolated on 2D gels. However, the advent of mass spectrometry-based proteomics protocols combined with genome information enabled us to determine the identity of a protein separated on 2D gels archived decades ago. The protocol will assist researchers to decipher molecular mechanisms involved in the system by identifying and quantifying the protein of interest from archived 2D gels.

Key words

Protein identification Archived 2D gels 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  • Hiroyuki Matsumoto
    • 1
  • Nobuaki Takemori
    • 2
  • Naoka Komori
    • 1
  1. 1.Department of Biochemistry and Molecular BiologyUniversity of Oklahoma Health Sciences CenterOklahoma CityUSA
  2. 2.Proteomics Core Laboratory, Proteo-Medicine Research CenterEhime UniversityToon CityJapan

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