Abstract
Genetic transformation plays a key role in deciphering regulation of agronomic traits at molecular level in rice, a model monocot cereal crop. Here we describe an efficient and fast protocol for producing transgenic japonica rice plants using the Agrobacterium-mediated transformation method. The protocol simplifies medium compositions and transformation steps and can be easily followed by a lab technician with little tissue culture experience. Using this protocol, we have transformed thousands of gene constructs in the past 10 years and edited hundreds of genes with the CRISPR-Cas9 system recently.
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Acknowledgment
This work was supported by the Innovation Program of Chinese Academy of Agricultural Sciences.
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Wu, C., Sui, Y. (2019). Efficient and Fast Production of Transgenic Rice Plants by Agrobacterium-Mediated Transformation. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_7
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DOI: https://doi.org/10.1007/978-1-4939-8778-8_7
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