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Agrobacterium tumefaciens-Mediated Transformation of Tomato

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Transgenic Plants

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1864))

Abstract

Tomato is both an important food crop and serves as a model plant species that is used for various research investigations including understanding gene function. Transformation is commonly utilized to facilitate these investigations in combination with all the extensive genetic and genomic resources available for tomato. The transformation protocol routinely used in our laboratory has been applied to many different tomato genotypes and relies on Agrobacterium tumefaciens infection of young cotyledon sections. We have used vector systems for overexpression, RNA interference for gene silencing, and CRISPR/Cas9 for genome editing. Vectors used to design gene constructs contained selectable marker genes that conferred resistance to kanamycin, hygromycin, and the herbicide component, bialaphos. The protocol we follow for Agrobacterium-mediated transformation of both cultivated and wild species of tomato is detailed in this chapter.

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Acknowledgments

Van Eck acknowledges the National Science Foundation Plant Genome Research Program (IOS-1732253) for support related to her research on genetic engineering and genome editing of tomato.

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Authors and Affiliations

  1. The Boyce Thompson Institute, Ithaca, NY, USA

    Joyce Van Eck, Patricia Keen & Michelle Tjahjadi

  2. Plant Breeding and Genetics Section, School of Integrative Plant Science, Cornell University, Ithaca, NY, USA

    Joyce Van Eck

Authors
  1. Joyce Van Eck
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  2. Patricia Keen
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  3. Michelle Tjahjadi
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Corresponding author

Correspondence to Joyce Van Eck .

Editor information

Editors and Affiliations

  1. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Sandeep Kumar

  2. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Pierluigi Barone

  3. Corteva Agriscienceâ„¢, Agriculture Division of DowDuPontâ„¢, Johnston, IA, USA

    Michelle Smith

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Van Eck, J., Keen, P., Tjahjadi, M. (2019). Agrobacterium tumefaciens-Mediated Transformation of Tomato. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_16

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  • DOI: https://doi.org/10.1007/978-1-4939-8778-8_16

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8777-1

  • Online ISBN: 978-1-4939-8778-8

  • eBook Packages: Springer Protocols

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