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Transgenic Plants pp 3–18Cite as

Repurposing Macromolecule Delivery Tools for Plant Genetic Modification in the Era of Precision Genome Engineering

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1864))

Abstract

Efficient delivery of macromolecules into plant cells and tissues is important for both basic research and biotechnology product applications. In transgenic research, the goal is to deliver DNA molecules into regenerable cells and stably integrate them into the genome. Over the past 40 years, many macromolecule delivery methods have been studied. To generate transgenic plants, particle bombardment and Agrobacterium-mediated transformation are the methods of choice for DNA delivery. The rapid advance of genome editing technologies has generated new requirements on large biomolecule delivery and at the same time reinvigorated the development of new transformation technologies. Many of the gene delivery options that have been studied before are now being repurposed for delivering genome editing machinery for various applications. This article reviews the major progress in the development of tools for large biomolecule delivery into plant cells in the new era of precision genome engineering.

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  1. Syngenta Crop Protection, LLC, Research Triangle Park, NC, USA

    Qiudeng Que, Mary-Dell M. Chilton, Sivamani Elumalai, Heng Zhong, Shujie Dong & Liang Shi

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  1. Qiudeng Que
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  2. Mary-Dell M. Chilton
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Correspondence to Qiudeng Que .

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  1. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Sandeep Kumar

  2. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Pierluigi Barone

  3. Corteva Agriscience™, Agriculture Division of DowDuPont™, Johnston, IA, USA

    Michelle Smith

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Que, Q., Chilton, MD.M., Elumalai, S., Zhong, H., Dong, S., Shi, L. (2019). Repurposing Macromolecule Delivery Tools for Plant Genetic Modification in the Era of Precision Genome Engineering. In: Kumar, S., Barone, P., Smith, M. (eds) Transgenic Plants. Methods in Molecular Biology, vol 1864. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8778-8_1

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  • DOI: https://doi.org/10.1007/978-1-4939-8778-8_1

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8777-1

  • Online ISBN: 978-1-4939-8778-8

  • eBook Packages: Springer Protocols

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