Abstract
DNA methylation is a conserved epigenetic modification of animal genomes, but genome methylation patterns appear surprisingly diverse in insects. Whole-genome bisulfite sequencing (WGBS) represents a sensitive and robust method for the characterization of genome-wide methylation patterns at single-base resolution. Here, we describe a step-by-step protocol for the generation and analysis of WGBS datasets using standard Illumina sequencing platforms. In comparison to whole-genome sequencing, WGBS has additional caveats that require particular attention and are highlighted in this chapter.
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Acknowledgments
We thank Günter Raddatz, Stephan Wolf, Nicolle Diessl, and the DKFZ Genomics and Proteomics Core Facility for helpful discussions.
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Gatzmann, F., Lyko, F. (2019). Whole-Genome Bisulfite Sequencing for the Methylation Analysis of Insect Genomes. In: Brown, S., Pfrender, M. (eds) Insect Genomics. Methods in Molecular Biology, vol 1858. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8775-7_11
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DOI: https://doi.org/10.1007/978-1-4939-8775-7_11
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