Abstract
The formation of longitudinal zonation patterns is important for normal root development and is regulated by the transcription factor PLETHORA (PLT). PLT proteins form a concentration gradient, and PLT protein levels determine root zonation. A peptide hormone root meristem growth factor (RGF) and its receptors (RGFRs) act as key regulators of root development by regulating the PLT protein expression pattern. Here, we describe a method for monitoring PLT protein gradient patterns in Arabidopsis with exogenous RGF treatment.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsSpringer Nature is developing a new tool to find and evaluate Protocols. Learn more
References
Dolan L, Janmaat K, Willemsen V, Linstead P, Poethig S, Roberts K, Scheres B (1993) Cellular organisation of the Arabidopsis thaliana root. Development 119(1):71–84
Aida M, Beis D, Heidstra R, Willemsen V, Blilou I, Galinha C, Nussaume L, Noh Y, Amasino R, Scheres B (2004) The PLETHORA genes mediate patterning of the Arabidopsis root stem cell niche. Cell 119:109–120
Galinha C, Hofhuis H, Luijten M, Willemsen V, Blilou I, Heidstra R, Scheres B (2007) PLETHORA proteins as dose-dependent master regulators of Arabidopsis root development. Nature 449:1053–1057
Mähönen A, Tusscher K, Siligato R, Smetana O, Díaz-Triviño S, Salojärvi J, Wachsman G, Prasad K, Heidstra R, Scheres B (2014) PLETHORA gradient formation mechanism separates auxin responses. Nature 515:125–129
Matsuzaki Y, Ogawa-Ohnishi M, Mori A, Matsubayashi Y (2010) Secreted peptide signals required for maintenance of root stem cell niche in Arabidopsis. Science 329:1065–1067
Shinohara H, Mori A, Yasue N, Sumida K, Matsubayashi Y (2016) Identification of three LRR-RKs involved in perception of root meristem growth factor in Arabidopsis. Proc Natl Acad Sci U S A 113:3897–3902
Acknowledgments
This research was supported by a Grants-in-Aid for Scientific Research on Innovative Areas (15H05957 to Y.M., 26113520 and 16H01234 to H.S.), a Grant-in-Aid for Scientific Research (S) (25221105 to Y.M.), and a Grant-in-Aid for Young Scientists (B) (25840111 to H.S.).
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Shinohara, H., Matsubayashi, Y. (2018). Analysis of PLETHORA Gradient Formation by Secreted Peptides During Root Development. In: Dubrulle, J. (eds) Morphogen Gradients. Methods in Molecular Biology, vol 1863. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8772-6_9
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8772-6_9
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8771-9
Online ISBN: 978-1-4939-8772-6
eBook Packages: Springer Protocols