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Morphogen Gradients pp 165–182Cite as

Live Imaging of mRNA Transcription in Drosophila Embryos

Part of the Methods in Molecular Biology book series (MIMB,volume 1863)

Abstract

Live imaging has been used in recent years for the understanding of dynamic processes in biology, such as embryo development. This was made possible by a combination of advancements in microscopy, leading to improved signal-to-noise ratios and better spatial and temporal resolutions, and by the development of new fluorescence markers, allowing for the quantification of protein expression and transcriptional dynamics in vivo. Here we describe a general protocol, which can be used in standard confocal microscopes to image early Drosophila melanogaster embryos, in order to learn about the transcriptional dynamics of a fluorescently labeled RNA.

Key words

  • MS2 system
  • Live imaging
  • Confocal microscopy
  • RNA
  • Embryo

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  • DOI: 10.1007/978-1-4939-8772-6_10
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Acknowledgments

The authors thank Patricia Le Baccon and the Imaging Facility PICT-IBiSA of the Institut Curie. This work was supported by a PSL IDEX REFLEX Grant for Mesoscopic Biology (ND, AMW, MC), an Ontario Trillium Scholarship for International Students (CAPR), a Mitacs Global Link Scholarship (CAPR) and an Internal Curie Institute Scholarship (CAPR), ARC PJA20151203341 (ND), a Mayent Rothschild sabbatical Grant from the Curie Institute (CF) and an NSERC discovery grant RGPIN/06362-15 (CF), a Marie Curie MCCIG grant No. 303561 (AMW), ANR-11-LABX-0044 DEEP Labex (ND), ANR- 11-BSV2-0024 Axomorph (ND and AMW) and PSL ANR-10-IDEX-0001-02. Cécile Fradin and Nathalie Dostatni contributed equally to this work. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Author notes

  1. Cécile Fradin and Nathalie Dostatni contributed equally to this work.

Authors and Affiliations

  1. Institut Curie, PSL Research University, CNRS, Sorbonne Université, Nuclear Dynamics, Paris, France

    Carmina Angelica Perez-Romero, Huy Tran, Cécile Fradin & Nathalie Dostatni

  2. McMaster University, Hamilton, ON, Canada

    Carmina Angelica Perez-Romero & Cécile Fradin

  3. Ecole Normale Supérieure, PSL Research University, CNRS, Sorbonne Université, Physique Théorique, Paris, France

    Huy Tran & Aleksandra M. Walczak

  4. Institut Curie, PSL Research University, CNRS, Sorbonne Université, Physico Chimie, Paris, France

    Mathieu Coppey

Authors
  1. Carmina Angelica Perez-Romero
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  2. Huy Tran
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  3. Mathieu Coppey
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  4. Aleksandra M. Walczak
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  5. Cécile Fradin
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  6. Nathalie Dostatni
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Corresponding author

Correspondence to Nathalie Dostatni .

Editor information

Editors and Affiliations

  1. Integrated Microscopy Core, Baylor College of Medicine, Houston, TX, USA

    Julien Dubrulle

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Perez-Romero, C.A., Tran, H., Coppey, M., Walczak, A.M., Fradin, C., Dostatni, N. (2018). Live Imaging of mRNA Transcription in Drosophila Embryos. In: Dubrulle, J. (eds) Morphogen Gradients. Methods in Molecular Biology, vol 1863. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8772-6_10

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  • DOI: https://doi.org/10.1007/978-1-4939-8772-6_10

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8771-9

  • Online ISBN: 978-1-4939-8772-6

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