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Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers

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Book cover Metabolic Signaling

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1862))

Abstract

Endothelial cells (ECs) form the inner lining of the vascular network. Although they can remain quiescent for years, ECs exhibit high plasticity in both physiological and pathological conditions, when they need to rapidly form new blood vessels in a process called angiogenesis. EC metabolism recently emerged as an important driver of this angiogenic switch. The use of radioactive tracer substrates to assess metabolic flux rates in ECs has been essential for the discovery that fatty acid, glucose, and glutamine metabolism critically contribute to vessel sprouting. In the future, these assays will be useful as a tool for the characterization of pathological conditions in which deregulation of EC metabolism underlies and/or precedes the disease, but also for the identification of anti-angiogenic metabolic targets. This chapter describes in detail the radioactive tracer substrate assays that have been used for the determination of EC metabolic flux in vitro.

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Author notes

  1. Koen Veys and Abdiel Alvarado-Diaz contributed equally to this work.

Authors and Affiliations

  1. Laboratory of Angiogenesis and Vascular Metabolism, VIB Center for Cancer Biology, VIB, Leuven, Belgium

    Koen Veys

  2. Laboratory of Angiogenesis and Vascular Metabolism, Department of Oncology, KU Leuven, Leuven, Belgium

    Koen Veys

  3. Laboratory of Exercise and Health, Department of Health Sciences and Technology, ETH Zürich, Zürich, Switzerland

    Abdiel Alvarado-Diaz & Katrien De Bock

Authors
  1. Koen Veys
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  2. Abdiel Alvarado-Diaz
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  3. Katrien De Bock
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Corresponding author

Correspondence to Katrien De Bock .

Editor information

Editors and Affiliations

  1. Laboratory of Cellular Metabolism and Metabolic Regulation, VIB-KU Leuven Center for Cancer Biology, Leuven, Belgium

    Sarah-Maria Fendt

  2. Department of Biochemistry and Molecular Biology, Michigan State University, East Lansing, MI, USA

    Sophia Y. Lunt

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Veys, K., Alvarado-Diaz, A., De Bock, K. (2019). Measuring Glycolytic and Mitochondrial Fluxes in Endothelial Cells Using Radioactive Tracers. In: Fendt, SM., Lunt, S. (eds) Metabolic Signaling. Methods in Molecular Biology, vol 1862. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8769-6_9

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  • DOI: https://doi.org/10.1007/978-1-4939-8769-6_9

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-8768-9

  • Online ISBN: 978-1-4939-8769-6

  • eBook Packages: Springer Protocols

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