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X-Chromosome Inactivation pp 205–219Cite as

X-Chromosome Inactivation and Escape from X Inactivation in Mouse

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1861))

Abstract

X chromosome inactivation silences one X chromosome in female mammals. However, this silencing is incomplete, and some genes escape X inactivation. We describe methods to determine the chromosome-wide X inactivation status of genes in tissues or cell lines derived from mice using a combination of skewing of X inactivation and allele-specific analyses of gene expression based on RNA-seq.

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Acknowledgments

This work was supported by grants GM046883 (C.M.D.), GM113943 (C.M.D., W.M.), and DK107979 (C.M.D., W.N.).

Author information

Authors and Affiliations

  1. Department of Statistics, University of California, Riverside, Riverside, CA, USA

    Wenxiu Ma

  2. Department of Genome Sciences, University of Washington, Seattle, WA, USA

    Giancarlo Bonora & William S. Noble

  3. Department of Pathology, University of Washington, Seattle, WA, USA

    Joel B. Berletch, Xinxian Deng & Christine M. Disteche

  4. Department of Medicine, University of Washington, Seattle, WA, USA

    Christine M. Disteche

Authors
  1. Wenxiu Ma
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  2. Giancarlo Bonora
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  3. Joel B. Berletch
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  4. Xinxian Deng
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  5. William S. Noble
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  6. Christine M. Disteche
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Corresponding author

Correspondence to Christine M. Disteche .

Editor information

Editors and Affiliations

  1. Department of Advanced Bioscience, Graduate School of Agriculture, Kindai University, Nara, Japan

    Takashi Sado

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Ma, W., Bonora, G., Berletch, J.B., Deng, X., Noble, W.S., Disteche, C.M. (2018). X-Chromosome Inactivation and Escape from X Inactivation in Mouse. In: Sado, T. (eds) X-Chromosome Inactivation. Methods in Molecular Biology, vol 1861. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-8766-5_15

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  • DOI: https://doi.org/10.1007/978-1-4939-8766-5_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-8765-8

  • Online ISBN: 978-1-4939-8766-5

  • eBook Packages: Springer Protocols

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